|CAT No.BT-CYT-260 |
| Vascular Endothelial Growth Factor Human Recombinant, CHO, VEGF Human, CHO, 10ug|
Determined by its ability to stimulate 3H-Thymidine incorporation in human ublical vein endothelial cells, the ED50 for this effect was found to be 2-6ng/ml corresponding to a Specific Activity of 166,667-500,000IU/mg.
Vascular Endothelial Growth Factor Human Recombinant produced in CHO cells is a double, glycosylated, polypeptide chain containing 165 amino acids and migrates as 44 kDa in SDS-PAGE under non-reducing conditions.
The VEGF is purified by proprietary chromatographic techniques.
The protein was lyophilized from a Phosphate- Buffered Saline, pH 7.4.
Sterile Filtered White lyophilized (freeze-dried) powder.
Greater than 97.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
It is recommended to reconstitute the lyophilized Vascular Endothelial Growth Factor Human in sterile 18M¦¸-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Chinese Hamster Ovarian Cell.
Lyophilized Vascular Endothelial Growth Factor Human although stable at room temperature for 3 weeks, should be stored desiccated below -18¡ãC. Upon reconstitution VEGF Human Recombinant should be stored at 4¡ãC between 2-7 days and for future use below -18¡ãC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Vascular endothelial growth factor A, VEGF-A, Vascular permeability factor, VPF, VEGF, MGC70609.
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